pFN11A (BIND) Flexi(R) Vector This vector can be obtained from Promega Corporation. Madison, WI. For ordering or technical information call one of the following numbers: Order or Technical: 1-800-356-9526 Outside U.S. 608-274-4330 pFN11A (BIND) Flexi(R) Vector sequence reference points: CMV intermediate/early enhancer/promoter 1-742 Chimeric intron 857-989 T7 RNA polymerase promoter (-17 to +3) 1033-1052 GAL4 fusion protein 1083-1520 IgA linker 1521-1553 Sgf I region 1554-1561 Barnase coding region 1585-1920 Pme I region 1922-1929 SV40 late polyadenylation signal 2081-2302 SV40 enhancer/early promoter 2401-2819 hGH intron 2871-3130 Renilla luciferase coding region 3155-4090 Synthetic polyadenylation signal 4148-4194 Beta-lactamase (Ampr) coding region 4457-5317 ColE1-derived plasmid origin of replication 5472-5508 cer region (for E. coli XerCD recombinase) 6179-6464 Note: The IgA linker encodes the polypeptide AIPSTPPTPSPAIA. 1 TCAATATTGG CCATTAGCCA TATTATTCAT TGGTTATATA GCATAAATCA 51 ATATTGGCTA TTGGCCATTG CATACGTTGT ATCTATATCA TAATATGTAC 101 ATTTATATTG GCTCATGTCC AATATGACCG CCATGTTGGC ATTGATTATT 151 GACTAGTTAT TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT 201 ATATGGAGTT CCGCGTTACA TAACTTACGG TAAATGGCCC GCCTGGCTGA 251 CCGCCCAACG ACCCCCGCCC ATTGACGTCA ATAATGACGT ATGTTCCCAT 301 AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG GAGTATTTAC 351 GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTCCG 401 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA 451 GTACATGACC TTACGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG 501 TCATCGCTAT TACCATGGTG ATGCGGTTTT GGCAGTACAC CAATGGGCGT 551 GGATAGCGGT TTGACTCACG GGGATTTCCA AGTCTCCACC CCATTGACGT 601 CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT CCAAAATGTC 651 GTAATAACCC CGCCCCGTTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 701 GAGGTCTATA TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCACTAGAAG 751 CTTTATTGCG GTAGTTTATC ACAGTTAAAT TGCTAACGCA GTCAGTGCTT 801 CTGACACAAC AGTCTCGAAC TTAAGCTGCA GAAGTTGGTC GTGAGGCACT 851 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA 901 ACTGGGCTTG TCGAGACAGA GAAGACTCTT GCGTTTCTGA TAGGCACCTA 951 TTGGTCTTAC TGACATCCAC TTTGCCTTTC TCTCCACAGG TGTCCACTCC 1001 CAGTTCAATT ACAGCTCTTA AGGCTAGAGT ATTAATACGA CTCACTATAG 1051 GGCTAGCCAG CTTGAAGCAA GCCTCCTGAA AGATGAAGCT ACTGTCTTCT 1101 ATCGAACAAG CATGCGATAT TTGCCGACTT AAAAAGCTCA AGTGCTCCAA 1151 AGAAAAACCG AAGTGCGCCA AGTGTCTGAA GAACAACTGG GAGTGTCGCT 1201 ACTCTCCCAA AACCAAAAGG TCTCCGCTGA CTAGGGCACA TCTGACAGAA 1251 GTGGAATCAA GGCTAGAAAG ACTGGAACAG CTATTTCTAC TGATTTTTCC 1301 TCGAGAAGAC CTTGACATGA TTTTGAAAAT GGATTCTTTA CAGGATATAA 1351 AAGCATTGTT AACAGGATTA TTTGTACAAG ATAATGTGAA TAAAGATGCC 1401 GTCACAGATA GATTGGCTTC AGTGGAGACT GATATGCCTC TAACATTGAG 1451 ACAGCATAGA ATAAGTGCGA CATCATCATC GGAAGAGAGT AGTAACAAAG 1501 GTCAAAGACA GTTGACTGTA GCGATTCCGT CGACACCACC TACTCCCTCT 1551 CCAGCGATCG CCATGGAATA AGTAAGGAAT CCACATGGCA CAGGTTATCA 1601 ACACGTTTGA CGGGGTTGCG GATTATCTTC AGACATATCA TAAGCTACCT 1651 GATAATTACA TTACAAAATC AGAAGCACAA GCCCTCGGCT GGGTGGCATC 1701 AAAAGGGAAC CTTGCAGACG TCGCTCCGGG GAAAAGCATC GGCGGAGACA 1751 TCTTCTCAAA CAGGGAAGGC AAACTCCCGG GCAAAAGCGG ACGAACATGG 1801 CGTGAAGCGG ATATTAACTA TACATCAGGC TTCAGAAATT CAGACCGGAT 1851 TCTTTACTCA AGCGACTGGC TGATTTACAA AACAACGGAC CATTATCAGA 1901 CCTTTACAAA AATCAGATAA TGTTTAAACG AATTCGGGCT CGGTACCCGG 1951 GGATCCTCTA GAGTCGACCT GCAGGCATGC AAGCTGATCC GGCTGCTAAC 2001 AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT 2051 AGCATAACCC CTTGGGGCGG CCGCTTCGAG CAGACATGAT AAGATACATT 2101 GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT 2151 TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA 2201 ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG 2251 GGGGAGATGT GGGAGGTTTT TTTAAGCAAG TAAAACCTCT ACAAATGTGG 2301 TAAAATCGAA TTTTAACAAA ATATTAACGC TTACAATTTC CTGATGCGGT 2351 ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ACGCGGATCT 2401 GCGCAGCACC ATGGCCTGAA ATAACCTCTG AAAGAGGAAC TTGGTTAGGT 2451 ACCTTCTGAG GCGGAAAGAA CCAGCTGTGG AATGTGTGTC AGTTAGGGTG 2501 TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC 2551 TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC 2601 AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC 2651 CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC 2701 CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC 2751 TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT 2801 AGGCTTTTGC AAAAAGCTTG ATTCTTCTGA CACAACAGTC TCGAACTTAA 2851 GGCTAGAATT CTGGCTACAG GTAAGCGCCC CTAAAATCCC TTTGGGCACA 2901 ATGTGTCCTG AGGGGAGAGG CAGCGACCTG TAGATGGGAC GGGGGCACTA 2951 ACCCTCAGGT TTGGGGCTTC TGAATGTGAG TATCGCCATG TAAGCCCAGT 3001 ATTTGGCCAA TCTCAGAAAG CTCCTGGTCC CTGGAGGGAT GGAGAGAGAA 3051 AAACAAACAG CTCCTGGAGC AGGGAGAGTG CTGGCCTCTT GCTCTCCGGC 3101 TCCCTCTGTT GCCCTCTGGT TTCTCCCCAG GCTCCCGGAC GTCCTCTAGC 3151 CACCATGACT TCGAAAGTTT ATGATCCAGA ACAAAGGAAA CGGATGATAA 3201 CTGGTCCGCA GTGGTGGGCC AGATGTAAAC AAATGAATGT TCTTGATTCA 3251 TTTATTAATT ATTATGATTC AGAAAAACAT GCAGAAAATG CTGTTATTTT 3301 TTTACATGGT AACGCGGCCT CTTCTTATTT ATGGCGACAT GTTGTGCCAC 3351 ATATTGAGCC AGTAGCGCGG TGTATTATAC CAGACCTTAT TGGTATGGGC 3401 AAATCAGGCA AATCTGGTAA TGGTTCTTAT AGGTTACTTG ATCATTACAA 3451 ATATCTTACT GCATGGTTTG AACTTCTTAA TTTACCAAAG AAGATCATTT 3501 TTGTCGGCCA TGATTGGGGT GCTTGTTTGG CATTTCATTA TAGCTATGAG 3551 CATCAAGATA AGATCAAAGC AATAGTTCAC GCTGAAAGTG TAGTAGATGT 3601 GATTGAATCA TGGGATGAAT GGCCTGATAT TGAAGAAGAT ATTGCGTTGA 3651 TCAAATCTGA AGAAGGAGAA AAAATGGTTT TGGAGAATAA CTTCTTCGTG 3701 GAAACCATGT TGCCATCAAA AATCATGAGA AAGTTAGAAC CAGAAGAATT 3751 TGCAGCATAT CTTGAACCAT TCAAAGAGAA AGGTGAAGTT CGTCGTCCAA 3801 CATTATCATG GCCTCGTGAA ATCCCGTTAG TAAAAGGTGG TAAACCTGAC 3851 GTTGTACAAA TTGTTAGGAA TTATAATGCT TATCTACGTG CAAGTGATGA 3901 TTTACCAAAA ATGTTTATTG AATCGGACCC AGGATTCTTT TCCAATGCTA 3951 TTGTTGAAGG TGCCAAGAAG TTTCCTAATA CTGAATTTGT CAAAGTAAAA 4001 GGTCTTCATT TTTCGCAAGA AGATGCACCT GATGAAATGG GAAAATATAT 4051 CAAATCGTTC GTTGAGCGAG TTCTCAAAAA TGAACAATAA TTCTAGCCCT 4101 GAATAAGTGA TAATAAGCGG ATGAATGGCA GAAATTCGTC GAAGCGCAAT 4151 AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGAATC 4201 GATAGCGATA AGGATCCTCT TTGCGCTTGC GTTTTCCCTT GTCCAGATAG 4251 CCCAGTAGCT GACATTCATC CGGGGTCAGC ACCGTTTCTG CGGACTGGCT 4301 TTCTACGTAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT 4351 GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC 4401 CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG 4451 AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC 4501 GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA 4551 AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT 4601 CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC 4651 AATGATGAGC ACTTTCAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA 4701 TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT 4751 GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT 4801 GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG 4851 CGGCCAACTT ACTTCTGACA ACTATCGGAG GACCGAAGGA GCTAACCGCT 4901 TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC 4951 GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG 5001 TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT 5051 CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC 5101 AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA 5151 AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG 5201 CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA 5251 GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC 5301 TGATTAAGCA TTGGTAATTC GAAATGACCG ACCAAGCGAC GCCCAACCGG 5351 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT 5401 AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 5451 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 5501 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA 5551 CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 5601 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 5651 GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG 5701 TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 5751 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 5801 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA 5851 GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 5901 CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 5951 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC 6001 GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 6051 AAAAGGATTT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 6101 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA 6151 AGGATCTTCA CCTAGATCCT TTTATAGTCC GGAAATACAG GAACGCACGC 6201 TGGATGGCCC TTCGCTGGGA TGGTGAAACC ATGAAAAATG GCAGCTTCAG 6251 TGGATTAAGT GGGGGTAATG TGGCCTGTAC CCTCTGGTTG CATAGGTATT 6301 CATACGGTTA AAATTTATCA GGCGCGATTG CGGCAGTTTT TCGGGTGGTT 6351 TGTTGCCATT TTTACCTGTC TGCTGCCGTG ATCGCGCTGA ACGCGTTTTA 6401 GCGGTGCGTA CAATTAAGGG ATTATGGTAA ATCCACTTAC TGTCTGCCCT 6451 CGTAGCCATC GAGATAAACC GCAGTACTCC GGCCACGATG CGTCCGGCGT 6501 AGAGGATCGA GATCT File updated 21-Jun-06.